Journal: Lab on a chip

Article Title: Microfluidic isolation of platelet-covered circulating tumor cells

doi: 10.1039/c7lc00654c

Figure Lengend Snippet: Lung CTC isolation using platelet- and EpCAM-targeted capture. (A) CTC counts in blood samples obtained from healthy donor (HD) and metastatic lung cancer patients (Lung pt) that were captured using CD41 or EpCAM antibodies. (B) Fluorescent images of CTCs captured using CD41 (top) and EpCAM antibodies (bottom). The cells were immunostained for EpCAM/cadherin-11 (CTC marker; Alexa Fluor 488; green), CD45 (leukocyte marker; Alexa Fluor 647; red) and CD61 (platelet marker; Alexa Fluor 568; gold) respectively. Scale bar, 10 μm.

Article Snippet: For lung and breast patient samples, captured cells on the HB-Chips were stained with fluorochrome-conjugated antibodies as follows: anti-EpCAM (3:100, Alexa Fluor 488 conjugate, Cell Signaling Technologies), anti-cadherin-11 (1:10, Alexa Fluor 488 conjugate, R&D Systems), anti-CD45 (1:20, Alexa Fluor 647 conjugate, BD Biosciences), and anti-CD61 (1:100, Abcam) that was conjugated to Alexa Fluor 568 via amine groups (Alexa Fluor 568 Antibody Labeling Kit; Life Technologies).

Techniques: Isolation, Marker

Journal: Lab on a chip

Article Title: Microfluidic isolation of platelet-covered circulating tumor cells

doi: 10.1039/c7lc00654c

Figure Lengend Snippet: (A) CTC count from breast cancer and melanoma patient blood using platelet-targeted capture. (B) Representative fluorescent images of CTCs from lung and breast cancer and melanoma patient samples. Lung and breast CTCs were immunostained for EpCAM/Cadherin-11 (Alexa Fluor 488; green). Melanoma CTCs were immunostained for CSPG4, MCAM, TYRP1, and α-SMA (Alexa Fluor 488; green). Leukocytes and platelets were stained with antibodies to CD45 (Alexa Fluor 647; red) and CD61 (Alexa Fluor 568; gold) respectively. Note the extensive platelet coating on both CTCs and leukocytes. Scale bar, 10 μm.

Article Snippet: For lung and breast patient samples, captured cells on the HB-Chips were stained with fluorochrome-conjugated antibodies as follows: anti-EpCAM (3:100, Alexa Fluor 488 conjugate, Cell Signaling Technologies), anti-cadherin-11 (1:10, Alexa Fluor 488 conjugate, R&D Systems), anti-CD45 (1:20, Alexa Fluor 647 conjugate, BD Biosciences), and anti-CD61 (1:100, Abcam) that was conjugated to Alexa Fluor 568 via amine groups (Alexa Fluor 568 Antibody Labeling Kit; Life Technologies).

Techniques: Staining

Journal: Lab on a chip

Article Title: Microfluidic isolation of platelet-covered circulating tumor cells

doi: 10.1039/c7lc00654c

Figure Lengend Snippet: CTC-leukocyte clusters. (A) CTC counts in the form of single cells and clusters that contain 1–2 or >2 leukocytes (WBCs). (B–D) Representative fluorescent images of CTC-leukocyte clusters from lung cancer patient samples. Cells were stained with antibodies to EpCAM/cadherin-11 (Alexa Fluor 488; green), CD45 (Alexa Fluor 647; red), and CD61 (gold). Scale bar, 10 μm.

Article Snippet: For lung and breast patient samples, captured cells on the HB-Chips were stained with fluorochrome-conjugated antibodies as follows: anti-EpCAM (3:100, Alexa Fluor 488 conjugate, Cell Signaling Technologies), anti-cadherin-11 (1:10, Alexa Fluor 488 conjugate, R&D Systems), anti-CD45 (1:20, Alexa Fluor 647 conjugate, BD Biosciences), and anti-CD61 (1:100, Abcam) that was conjugated to Alexa Fluor 568 via amine groups (Alexa Fluor 568 Antibody Labeling Kit; Life Technologies).

Techniques: Staining

Journal:

Article Title: Cyclophosphamide Increases Transgene Expression Mediated by an Oncolytic Adenovirus in Glioma-Bearing Mice Monitored by Bioluminescence Imaging

doi: 10.1016/j.ymthe.2006.08.008

Figure Lengend Snippet: Immunohistochemical staining for common leukocyte antigen (CD45), monocytic cells/macrophages (CD68), and adenoviral hexon proteins in intracranial U-87ΔEGFR tumors of athymic mice treated with AdΔ24CMV-Luc+PBS (top) or AdΔ24CMV-Luc+CPA (bottom) at (A) 72 h postinfection or (B) 5 days postinfection. Original magnification ×20.

Article Snippet: Briefly, immunohistochemical staining was performed for adenovirus using the goat antiadenoviral hexon protein antibody 1056 (Chemicon Europe, Hampshire, UK), for monocytes/macrophages using the rat anti-mouse CD68 antibody (Serotec, Oxford, UK), and for hematopoietic cells using the rat anti-mouse CD45 antibody to leukocyte common antigen (ITK, Uithoorn, The Netherlands).

Techniques: Immunohistochemical staining, Staining

Journal:

Article Title: Cyclophosphamide Increases Transgene Expression Mediated by an Oncolytic Adenovirus in Glioma-Bearing Mice Monitored by Bioluminescence Imaging

doi: 10.1016/j.ymthe.2006.08.008

Figure Lengend Snippet: (A) Quantitative image analysis of intensity of light emitted from intracranial tumor sites in NOD/SCID mice showing transgene expression levels of mice treated with CPA+ AdΔ24CMV-Luc (CPA) or PBS+AdΔ24CMV-Luc (Control). Shown are mean total relative light units (RLU) per mouse±SEM. (B) Immunohistochemical staining for CD45, CD68, and adenoviral hexon proteins in intracranial U-87ΔEGFR tumors of NOD/SCID mice treated with AdΔ24CMV-Luc+PBS (top) or AdΔ24CMV-Luc+CPA (bottom). Original magnification × 20.

Article Snippet: Briefly, immunohistochemical staining was performed for adenovirus using the goat antiadenoviral hexon protein antibody 1056 (Chemicon Europe, Hampshire, UK), for monocytes/macrophages using the rat anti-mouse CD68 antibody (Serotec, Oxford, UK), and for hematopoietic cells using the rat anti-mouse CD45 antibody to leukocyte common antigen (ITK, Uithoorn, The Netherlands).

Techniques: Expressing, Immunohistochemical staining, Staining